[Microbiological characteristics of a Lysinibacillus strain isolated from Populous euphratica].
Identifieur interne : 001A46 ( Main/Exploration ); précédent : 001A45; suivant : 001A47[Microbiological characteristics of a Lysinibacillus strain isolated from Populous euphratica].
Auteurs : Nusratgul Anwar ; Raziya Erkin ; Hormathan Mamtimin ; Buayshem Hamood ; Nurimangul Ismayi ; Muhtar Abdukerim ; Erkin RahmanSource :
- Wei sheng wu xue bao = Acta microbiologica Sinica [ 0001-6209 ] ; 2015.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), ARN ribosomique 16S (génétique), Acides gras (métabolisme), Bacillaceae (classification), Bacillaceae (génétique), Bacillaceae (isolement et purification), Bacillaceae (métabolisme), Composition en bases nucléiques (MeSH), Données de séquences moléculaires (MeSH), Phylogenèse (MeSH), Populus (microbiologie), Techniques de typage bactérien (MeSH).
- MESH :
- génétique : ADN bactérien, ARN ribosomique 16S, Bacillaceae.
- isolement et purification : Bacillaceae.
- microbiologie : Populus.
- métabolisme : Acides gras, Bacillaceae.
- Composition en bases nucléiques, Données de séquences moléculaires, Phylogenèse, Techniques de typage bactérien.
English descriptors
- KwdEn :
- Bacillaceae (classification), Bacillaceae (genetics), Bacillaceae (isolation & purification), Bacillaceae (metabolism), Bacterial Typing Techniques (MeSH), Base Composition (MeSH), DNA, Bacterial (genetics), Fatty Acids (metabolism), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Populus (microbiology), RNA, Ribosomal, 16S (genetics).
- MESH :
- chemical , genetics : DNA, Bacterial, RNA, Ribosomal, 16S.
- classification : Bacillaceae.
- genetics : Bacillaceae.
- isolation & purification : Bacillaceae.
- metabolism : Bacillaceae, Fatty Acids.
- microbiology : Populus.
- Bacterial Typing Techniques, Base Composition, Molecular Sequence Data, Phylogeny.
Abstract
OBJECTIVE
The aim of this study was to identify the microbiological characteristics of a Lysinibacillus strain isolated from storage liquid in the stems of Populus euphratica trees.
METHODS
Bacterial morphology and cultivation characteristics were studied by conventional cultivation and dyeing method. Biochemical characteristics, fatty acid components, menaquinone, polar lipids, phylogenetic analyses of 16S rRNA, determination of (G + C) mol% content and DNA- DNA hybridization were studied by polyphasic taxonomic approach.
RESULTS
Strain ML-64 is Gram-positive, endospore-forming and rod-shaped. Colonies are pale-yellow, circular and entire margin. Temperature range for growth is between 10 and 45 degrees C (optimum at 37 degrees C ). The pH range for growth is between 6. 0 and 9.0 (optimum at 7.0). NaCl concentration range for growth is between 0 and 6% (optimum 3% ). Cells were positive for lipid esterase, Arginine dihydrolase, urease and Voges-Proskauer test. No sugars were fermented in the API 50CH strips. L-Serine, Methyl Pyruvate, α-Keto-Butyric, Acetoacetic Acid were oxidized. Resistant to polymyxin b (30 μg), novobiocin (30 μg), peillin G (10 U). 16S rRNA gene sequence demonstrated that strain ML-64 was closely related to Lysinibacillus chungkukjangi 2RL3-2T (100%) , Lysinibacillus sinduriensis BLB-1T (99.1%). DNA-DNA relatedness were 82% and 50. 9% with Lysinibacillus chungkukjangi 2RL3-2T and Lysinibacillus massiliensis CIP108446T, respectively. The genomic DNA G + C content of strain ML-64 was 36. 8% (mol). Major fatty acids were iso-C,,, (55. 05% ) and anteiso-C15,0 (20. 70% ). The predominant menaquinone is MK-7. Based on the phenotypic phylogenetic and genotypic analyses, the strain ML-64 is concluded to represent a new mutant strain of the Lysinibacillus chungkukjangi species, GenBank accession number is KC609752.
CONCLUSION
As an endophytic bacterium of Populus euphratica, genomic structure of the strain ML-64 was greatly differentiated from the closest strain L. chungkukjangi, and suitably adapted to the endophytic environment of Populus euphratica.
PubMed: 26762029
Affiliations:
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first="Muhtar" last="Abdukerim">Muhtar Abdukerim</name></author><author><name sortKey="Rahman, Erkin" sort="Rahman, Erkin" uniqKey="Rahman E" first="Erkin" last="Rahman">Erkin Rahman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26762029</idno><idno type="pmid">26762029</idno><idno type="wicri:Area/Main/Corpus">001964</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001964</idno><idno type="wicri:Area/Main/Curation">001964</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001964</idno><idno type="wicri:Area/Main/Exploration">001964</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Microbiological characteristics of a Lysinibacillus strain isolated from Populous euphratica].</title><author><name sortKey="Anwar, Nusratgul" sort="Anwar, Nusratgul" uniqKey="Anwar N" first="Nusratgul" last="Anwar">Nusratgul Anwar</name></author><author><name sortKey="Erkin, Raziya" sort="Erkin, Raziya" uniqKey="Erkin R" first="Raziya" last="Erkin">Raziya Erkin</name></author><author><name sortKey="Mamtimin, Hormathan" sort="Mamtimin, Hormathan" uniqKey="Mamtimin H" first="Hormathan" last="Mamtimin">Hormathan Mamtimin</name></author><author><name sortKey="Hamood, Buayshem" sort="Hamood, Buayshem" uniqKey="Hamood B" first="Buayshem" last="Hamood">Buayshem Hamood</name></author><author><name sortKey="Ismayi, Nurimangul" sort="Ismayi, Nurimangul" uniqKey="Ismayi N" first="Nurimangul" last="Ismayi">Nurimangul Ismayi</name></author><author><name sortKey="Abdukerim, Muhtar" sort="Abdukerim, Muhtar" uniqKey="Abdukerim M" first="Muhtar" last="Abdukerim">Muhtar Abdukerim</name></author><author><name sortKey="Rahman, Erkin" sort="Rahman, Erkin" uniqKey="Rahman E" first="Erkin" last="Rahman">Erkin Rahman</name></author></analytic><series><title level="j">Wei sheng wu xue bao = Acta 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purification)</term><term>Bacillaceae (métabolisme)</term><term>Composition en bases nucléiques (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Populus (microbiologie)</term><term>Techniques de typage bactérien (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bacillaceae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillaceae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>ARN ribosomique 16S</term><term>Bacillaceae</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacillaceae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bacillaceae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillaceae</term><term>Fatty Acids</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides gras</term><term>Bacillaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Base Composition</term><term>Molecular Sequence Data</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Composition en bases nucléiques</term><term>Données de séquences moléculaires</term><term>Phylogenèse</term><term>Techniques de typage bactérien</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>The aim of this study was to identify the microbiological characteristics of a Lysinibacillus strain isolated from storage liquid in the stems of Populus euphratica trees.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Bacterial morphology and cultivation characteristics were studied by conventional cultivation and dyeing method. Biochemical characteristics, fatty acid components, menaquinone, polar lipids, phylogenetic analyses of 16S rRNA, determination of (G + C) mol% content and DNA- DNA hybridization were studied by polyphasic taxonomic approach.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Strain ML-64 is Gram-positive, endospore-forming and rod-shaped. Colonies are pale-yellow, circular and entire margin. Temperature range for growth is between 10 and 45 degrees C (optimum at 37 degrees C ). The pH range for growth is between 6. 0 and 9.0 (optimum at 7.0). NaCl concentration range for growth is between 0 and 6% (optimum 3% ). Cells were positive for lipid esterase, Arginine dihydrolase, urease and Voges-Proskauer test. No sugars were fermented in the API 50CH strips. L-Serine, Methyl Pyruvate, α-Keto-Butyric, Acetoacetic Acid were oxidized. Resistant to polymyxin b (30 μg), novobiocin (30 μg), peillin G (10 U). 16S rRNA gene sequence demonstrated that strain ML-64 was closely related to Lysinibacillus chungkukjangi 2RL3-2T (100%) , Lysinibacillus sinduriensis BLB-1T (99.1%). DNA-DNA relatedness were 82% and 50. 9% with Lysinibacillus chungkukjangi 2RL3-2T and Lysinibacillus massiliensis CIP108446T, respectively. The genomic DNA G + C content of strain ML-64 was 36. 8% (mol). Major fatty acids were iso-C,,, (55. 05% ) and anteiso-C15,0 (20. 70% ). The predominant menaquinone is MK-7. Based on the phenotypic phylogenetic and genotypic analyses, the strain ML-64 is concluded to represent a new mutant strain of the Lysinibacillus chungkukjangi species, GenBank accession number is KC609752.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>As an endophytic bacterium of Populus euphratica, genomic structure of the strain ML-64 was greatly differentiated from the closest strain L. chungkukjangi, and suitably adapted to the endophytic environment of Populus euphratica.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26762029</PMID><DateCompleted><Year>2016</Year><Month>03</Month><Day>14</Day></DateCompleted><DateRevised><Year>2016</Year><Month>01</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-6209</ISSN><JournalIssue CitedMedium="Print"><Volume>55</Volume><Issue>9</Issue><PubDate><Year>2015</Year><Month>Sep</Month><Day>04</Day></PubDate></JournalIssue><Title>Wei sheng wu xue bao = Acta microbiologica Sinica</Title><ISOAbbreviation>Wei Sheng Wu Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Microbiological characteristics of a Lysinibacillus strain isolated from Populous euphratica].</ArticleTitle><Pagination><MedlinePgn>1160-70</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">The aim of this study was to identify the microbiological characteristics of a Lysinibacillus strain isolated from storage liquid in the stems of Populus euphratica trees.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Bacterial morphology and cultivation characteristics were studied by conventional cultivation and dyeing method. Biochemical characteristics, fatty acid components, menaquinone, polar lipids, phylogenetic analyses of 16S rRNA, determination of (G + C) mol% content and DNA- DNA hybridization were studied by polyphasic taxonomic approach.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Strain ML-64 is Gram-positive, endospore-forming and rod-shaped. Colonies are pale-yellow, circular and entire margin. Temperature range for growth is between 10 and 45 degrees C (optimum at 37 degrees C ). The pH range for growth is between 6. 0 and 9.0 (optimum at 7.0). NaCl concentration range for growth is between 0 and 6% (optimum 3% ). Cells were positive for lipid esterase, Arginine dihydrolase, urease and Voges-Proskauer test. No sugars were fermented in the API 50CH strips. L-Serine, Methyl Pyruvate, α-Keto-Butyric, Acetoacetic Acid were oxidized. Resistant to polymyxin b (30 μg), novobiocin (30 μg), peillin G (10 U). 16S rRNA gene sequence demonstrated that strain ML-64 was closely related to Lysinibacillus chungkukjangi 2RL3-2T (100%) , Lysinibacillus sinduriensis BLB-1T (99.1%). DNA-DNA relatedness were 82% and 50. 9% with Lysinibacillus chungkukjangi 2RL3-2T and Lysinibacillus massiliensis CIP108446T, respectively. The genomic DNA G + C content of strain ML-64 was 36. 8% (mol). Major fatty acids were iso-C,,, (55. 05% ) and anteiso-C15,0 (20. 70% ). The predominant menaquinone is MK-7. Based on the phenotypic phylogenetic and genotypic analyses, the strain ML-64 is concluded to represent a new mutant strain of the Lysinibacillus chungkukjangi species, GenBank accession number is KC609752.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">As an endophytic bacterium of Populus euphratica, genomic structure of the strain ML-64 was greatly differentiated from the closest strain L. chungkukjangi, and suitably adapted to the endophytic environment of Populus euphratica.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Anwar</LastName><ForeName>Nusratgul</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Erkin</LastName><ForeName>Raziya</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Mamtimin</LastName><ForeName>Hormathan</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Hamood</LastName><ForeName>Buayshem</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Ismayi</LastName><ForeName>Nurimangul</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Abdukerim</LastName><ForeName>Muhtar</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Rahman</LastName><ForeName>Erkin</ForeName><Initials>E</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Wei Sheng Wu Xue Bao</MedlineTA><NlmUniqueID>21610860R</NlmUniqueID><ISSNLinking>0001-6209</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005227">Fatty 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MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005227" MajorTopicYN="N">Fatty Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26762029</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Abdukerim, Muhtar" sort="Abdukerim, Muhtar" uniqKey="Abdukerim M" first="Muhtar" last="Abdukerim">Muhtar Abdukerim</name><name sortKey="Anwar, Nusratgul" sort="Anwar, Nusratgul" uniqKey="Anwar N" first="Nusratgul" last="Anwar">Nusratgul Anwar</name><name sortKey="Erkin, Raziya" sort="Erkin, Raziya" uniqKey="Erkin R" first="Raziya" last="Erkin">Raziya Erkin</name><name sortKey="Hamood, Buayshem" sort="Hamood, Buayshem" uniqKey="Hamood B" first="Buayshem" last="Hamood">Buayshem Hamood</name><name sortKey="Ismayi, Nurimangul" sort="Ismayi, Nurimangul" uniqKey="Ismayi N" first="Nurimangul" last="Ismayi">Nurimangul Ismayi</name><name sortKey="Mamtimin, Hormathan" sort="Mamtimin, Hormathan" uniqKey="Mamtimin H" first="Hormathan" last="Mamtimin">Hormathan Mamtimin</name><name sortKey="Rahman, Erkin" sort="Rahman, Erkin" uniqKey="Rahman E" first="Erkin" last="Rahman">Erkin Rahman</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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